# Changes in Levels of Endocannabinoidome Mediators in Mice with Cancer Cachexia > **Author:** Benjamin Caplan, MD (ABFM Board Certified, MA Lic. #240562, NPI: 1962722637) > **Publisher:** CED Clinic (https://cedclinic.com) > **Canonical URL:** https://cedclinic.com/endocannabinoid-system-cancer-cachexia-mediators-changes/ > **Published:** 2026-03-26T11:07:39-04:00 | **Last Modified / Clinically Audited:** 2026-09-28T10:37:49-04:00 > **Clinical Summary:** Explore how the endocannabinoid system cancer cachexia mediators change in mice, linking metabolic dysfunction with gut microbiome dysbiosis and steatosis. --- CED Clinical Relevance  #56Monitored Relevance  Early-stage or contextual signal requiring further evidence before action. 🔬 Evidence Watch  |  CED Clinic CachexiaCancerEndocannabinoidPreclinicalMetabolism Journal BJC reports Study Type [Clinical Study](https://cedclinic.com/cannabis-hyperemesis-syndrome-psychiatric-risk/) Population Human participants Why This Matters Cancer cachexia affects up to 80% of advanced cancer patients and contributes significantly to mortality, yet therapeutic options remain limited. This preclinical study identifies specific alterations in the [endocannabinoid system](https://cedclinic.com/endocannabinoid-system-fear-extinction/) during cachexia development, potentially revealing new therapeutic targets for cannabis-based interventions. Clinical Summary Researchers used a mouse model of colon cancer cachexia (C26 cells) to examine changes in [endocannabinoid system](https://cedclinic.com/endocannabinoid-system-fear-extinction/) mediators in liver and intestinal tissues using mass spectrometry. The study found significant alterations in endocannabinoid concentrations that correlated with weight loss, liver fat accumulation, and gut microbiome disruption 10 days after cancer cell injection. This preclinical work maps the endocannabinoidome changes that occur during cachexia progression, linking metabolic dysfunction with gut microbiome alterations. However, the study is limited by its animal model design and relatively short observation period. Dr. Caplan’s Take “While this mechanistic research advances our understanding of how the endocannabinoid system changes during cancer cachexia, it doesn’t immediately change my clinical approach. I continue to see cannabis as potentially beneficial for cachexia symptoms like appetite loss and nausea, though we still lack robust human data on optimal dosing and cannabinoid ratios for this indication.” Clinical Perspective 🧠 Clinicians should recognize this as foundational research that may inform future cannabis-based cachexia treatments rather than immediate practice changes. For patients with cancer cachexia, cannabis remains a reasonable consideration for symptom management, particularly [appetite stimulation](https://cedclinic.com/dronabinol-hiv-wasting-syndrome-appetite-weight-gain/) and nausea control, while we await human studies that build on these mechanistic insights. 💬 Join the Conversation Have a question about how this applies to your situation? 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Share it: [𝕏 Share on X](https://twitter.com/intent/tweet?text=Just%20read%20this%20on%20CEDclinic.com%3A%20Changes%20in%20levels%20of%20endocannabinoidome%20mediators%20in%20mice%20with%20cancer%20cachexia%3A%20...%20%40drcaplan&url=https%3A//pubmed.ncbi.nlm.nih.gov/41781471/)[in Share on LinkedIn](https://www.linkedin.com/sharing/share-offsite/?url=https%3A//pubmed.ncbi.nlm.nih.gov/41781471/)[🦥 Share on BlueSky](https://bsky.app/intent/compose?text=Just%20read%20this%3A%20Changes%20in%20levels%20of%20endocannabinoidome%20mediators%20in%20mice%20with%20cancer%20cachexia%3A%20...%20--%20via%20%40drcaplan.bsky.social%20https%3A//pubmed.ncbi.nlm.nih.gov/41781471/)[📷 Follow on Instagram](https://www.instagram.com/drbenjamincaplan/)[📝 Read more on Substack](https://substack.com/@doctorapproved)[🔔 Subscribe via RSS](https://cedclinic.com/category/cannabis-news/feed/) 📰 Source: [https://pubmed.ncbi.nlm.nih.gov/41781471/](https://pubmed.ncbi.nlm.nih.gov/41781471/) Table of Contents - [FAQ](#FAQ)[What is cancer cachexia and how does it relate to the endocannabinoid system?](#What_is_cancer_cachexia_and_how_does_it_relate_to_the_endocannabinoid_system) - [Could endocannabinoid-based treatments help manage cancer cachexia?](#Could_endocannabinoid-based_treatments_help_manage_cancer_cachexia) - [How does cancer cachexia affect liver function and fat metabolism?](#How_does_cancer_cachexia_affect_liver_function_and_fat_metabolism) - [What role does gut bacteria play in cancer cachexia?](#What_role_does_gut_bacteria_play_in_cancer_cachexia) - [Are there currently approved cannabis treatments for cancer cachexia?](#Are_there_currently_approved_cannabis_treatments_for_cancer_cachexia) - [Related](#Related) ## FAQ #### What is cancer cachexia and how does it relate to the endocannabinoid system? Cancer cachexia is a debilitating syndrome characterized by involuntary weight loss that commonly occurs in cancer patients. This study demonstrates that cachexia involves alterations in the endocannabinoidome (eCBome), a complex signaling system that includes endocannabinoids like anandamide and 2-AG, which normally help regulate energy balance and metabolism. #### Could endocannabinoid-based treatments help manage cancer cachexia? While this preclinical study shows that endocannabinoid signaling is disrupted in cancer cachexia, it represents early-stage research requiring further evidence. The findings suggest potential therapeutic targets, but clinical applications would need extensive human trials to establish safety and efficacy for [cachexia management](https://cedclinic.com/cannabis-appetite-effects-benefit-no-appetite/). #### How does cancer cachexia affect liver function and fat metabolism? The study found that cancer cachexia causes hepatic dyslipidemia and steatosis (fatty liver), alongside disrupted endocannabinoid signaling in liver tissue. These metabolic disruptions appear to be interconnected, as the endocannabinoid system normally plays a crucial role in regulating lipid metabolism and energy balance. #### What role does gut bacteria play in cancer cachexia? The research reveals that cancer cachexia involves gut microbiome dysbiosis, which appears linked to altered endocannabinoid signaling. This suggests a complex interaction between the endocannabinoid system, intestinal bacteria, and metabolic dysfunction in cachexia development. #### Are there currently approved cannabis treatments for cancer cachexia? While some cannabis-based medications are approved for [appetite stimulation](https://cedclinic.com/dronabinol-hiv-wasting-syndrome-appetite-weight-gain/) in cancer patients, this specific research on endocannabinoid system disruptions in cachexia is still in preclinical stages. Any therapeutic applications based on these findings would require substantial additional research and [clinical trials](https://cedclinic.com/endocannabinoid-system-emotional-memory-ptsd-treatment/) before potential medical use. {“@context”: “https://schema.org”, “@type”: “ScholarlyArticle”, “headline”: “Changes in levels of endocannabinoidome mediators in mice with cancer cachexia: links with steatosis and gut microbial dysbiosis.”, “url”: “https://pubmed.ncbi.nlm.nih.gov/41781471/”, “about”: “bjc reports [clinical study](https://cedclinic.com/cannabis-hyperemesis-syndrome-psychiatric-risk/) changes levels”, “isPartOf”: “BJC reports”} [https://cedclinic.com/contact/](https://cedclinic.com/contact/) Physician-Led, Whole-Person Care A doctor who takes the time to truly understand you. Personal care that starts with listening and is guided by experience and ingenuity. 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[Read More](https://cedclinic.com/anticancer-potential-cannabidiol-renal-cell-carcinoma/) [Progressive endocannabinoid system dysregulation in autosomal dominant polycystic kidney disease.](https://cedclinic.com/endocannabinoid-system-dysfunction-adpkd/) This article discusses how progressive dysregulation of the endocannabinoid system impacts autosomal dominant polycystic kidney disease, highlighting systemic effects. [Learn More](https://cedclinic.com/endocannabinoid-system-dysfunction-adpkd/) [Lifestyle and Behavioral Enhancements of Sleep: A Review.](https://cedclinic.com/sleep-hygiene-cbt-i-lifestyle-enhancements/) Review lifestyle and behavioral strategies to improve sleep quality, relevant for patients experiencing metabolic and systemic disruptions. [Discover More](https://cedclinic.com/sleep-hygiene-cbt-i-lifestyle-enhancements/) ### Related --- ### Verification & Medical Governance - **Lead Clinical Author:** Benjamin Caplan, MD - **Credentials:** Board Certified in Family Medicine (ABFM), Former CMO Canna Care Docs (250,000+ patients supervised), Founder & CMO CED Clinic & CED Foundation, Author of *The Doctor-Approved Cannabis Handbook* (Penguin Random House). - **Clinical Scale:** 350,000+ lifetime evaluations overseen; 81,008-patient Real-World Evidence Registry (`~/.ced_rwe_vault.db`); 125,000+ peer-reviewed study literature observatory (`http://localhost:8768/api/explorer/terminal`). - **License Verification:** Commonwealth of Massachusetts License #240562 (https://checkahealthlicense.mass.gov/). - **NPI Registry:** 1962722637 (https://npiregistry.cms.hhs.gov/provider-view/1962722637). - **Ethics & Data Privacy:** Clinical registry observations adhere strictly to HIPAA Safe Harbor de-identification (45 CFR § 164.514(b)) and criteria for secondary research exemption under 45 CFR § 46.104(d)(4).